包装
1000, 3000, 5000, 15000 units in serum bottle
Unit Definition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol ａnd D-galactose per min at pH 7.3 at 37 °C.
Physical form
Contains Tris buffer salts ａnd magnesium chloride
Application
β-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA).[1] β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.[2] It may be used as a positive control protein with anti-β-galactosidase antibodies.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose ａnd galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
General description
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.[3]
Physical properties
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
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